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Image Search Results
Journal: Journal of Virology
Article Title: Chicken Organic Anion-Transporting Polypeptide 1A2, a Novel Avian Hepatitis E Virus (HEV) ORF2-Interacting Protein, Is Involved in Avian HEV Infection
doi: 10.1128/JVI.02205-18
Figure Lengend Snippet: Designation, prediction, expression, and identification of soluble GST-ap237 in bacteria. (A) Amino acid alignments of genotype 3 human and avian HEV truncated capsid proteins. The alignments of capsid proteins from genotype 3 HEV and CaHEV isolates were performed using the Clustal W module of the MegAlign program of Lasergene 7.1 (DNASTAR, Inc.). (B) 3D structure of the CaHEV capsid protein predicted by using SWISS-MODEL and visualized by using CHIMERA software. The backbone of ap237 (aa 313 to 549) is indicated in green in the pentamer (left). The P domain (blue), M domain (dark blue), S domain (purple), and proline-rich hinge (green) of the monomer are highlighted with different colors. (C) SDS-PAGE of GST-ap237 protein produced in E. coli Transetta(DE3) cells. (D) Western blot analysis of the expression of recombinant GST-ap237 using anti-GST antibody. A GST tag expressed by empty vector pGEX-6P-1 was used as a negative control. (E) Western blot analysis of the expression of recombinant GST-ap237 using the 3E8 mAb. The vertical lines in panels D and E indicate marker (M) images; the Western blot results were taken separately and joined together.
Article Snippet: The alignments of capsid proteins from genotype 3 HEV and CaHEV isolates were performed using the Clustal W module of the
Techniques: Expressing, Bacteria, Software, SDS Page, Produced, Western Blot, Recombinant, Plasmid Preparation, Negative Control, Marker